Anti-Respiratory Syncytial Virus antibody ab20745 from Abcam

Product Specs
ItemAnti-Respiratory Syncytial Virus antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab20745
Antibody TypePrimary
IsotypeIgG
HostGoat
ClonalityPolyclonal
ApplicationsELISA, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity100 UL
Conjugate/TagUnconjugated
FormatSodium sulphate precipitation and DEAE chromatography
Concentration4 mg/ml
Gene NameM2-1
ReactivityRespiratory syncytial virus
TargetRespiratory Syncytial Virus

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) APOBEC3G Is a p53-Dependent Restriction Factor in Respiratory Syncytial Virus Infection of Human Cells Included in the p53/Immune AxisInternational Journal of Molecular SciencesNovember 27, 2023Wesley Gladwell, Oriana Yost, Heather Li, Whitney J. Bell, Shih-Heng Chen, James M. Ward, Steven R. Kleeberger, Michael A. Resnick, Daniel Menendezprimary antibodies used: p53 (DO-1), Actin (C-11), Lamin B1 (G-1) from Santa Cruz Biotechnology, RSV (ab20745 Abcam), anti-DDK (FLAG, Clone OTI4C5, Origene, Rockville, MD, USA), and turboGFP (OTI2H8, Origene). 4.8.

(2) Organisation of the orthobunyavirus tripodal spike and the structural changes induced by low pH and K+ during entryNature CommunicationsSeptember 21, 2023Samantha Hover, Frank W. Charlton, Jan Hellert, Jessica J. Swanson, Jamel Mankouri, John N. Barr, Juan FontanaAriza et al. (2013)44; and used extensively in previous publications18); goat α-HRSV (1:1000; Abcam ab20745); mouse α-GAPDH (1:2000, Santa Cruz sc47724); or mouse α-GFP (1:1000; Santa Cruz sc-9996). This was

(3) Antagonism between viral infection and innate immunity at the single-cell levelPLoS pathogensSeptember 1, 2023Grabowski F, Kochańczyk M, Korwek Z, Czerkies M, Prus W, Lipniacki T.number sc-376455; Santa Cruz Biotechnology; 1:500), anti-Respiratory Syncytial Virus (catalog number ab20745; Abcam; 1:1000), anti-human interferon beta (catalog number MAB8142; R&D Systems; 1:100). Secondary

(4) Human T cells efficiently control RSV infectionJCI InsightJune 8, 2023Chandrav De, Raymond J. Pickles, Wenbo Yao, Baolin Liao, Allison E Boone, Mingyu Choi, Diana M. Battaglia, Frederic B. Askin, Jason K. Whitmire, Guido Silvestri, et al.Laboratories). Polyclonal goat antibody directed against all RSV proteins was used to detect RSV antigen (Abcam, Ab20745). Tissue sections were developed using the ImmPRESS HRP Horse Anti-Goat IgG peroxidase Polymer Detection

(5) Respiratory Syncytial Virus Matrix Protein Is Sufficient and Necessary to Remodel Host Mitochondria in InfectionCellsMay 4, 2023MengJie Hu, Marie A. Bogoyevitch, David A. Jansstaining, as described previously [20]. The primary antibodies used were anti-RSV antibody (1:400, ab20745, Abcam, Cambridge, UK), anti-FLAG antibody (1:100, F1804, Sigma, St. Louis, MO, USA) with dye-tagged

(6) Respiratory Syncytial Virus Protects Bystander Cells against Influenza A Virus Infection by Triggering Secretion of Type I and Type III InterferonsJournal of VirologyNovember 23, 2022Maciej Czerkies, Marek Kochańczyk, Zbigniew Korwek, Wiktor Prus, Tomasz Lipniackinumber sc-376455; Santa Cruz Biotechnology) (1:500), anti-respiratory syncytial virus (catalog number ab20745; Abcam) (1:1,000), anti-influenza A virus (catalog number ab20841; Abcam) (1:1,000), anti-influenza A virus nucleoprotein

(7) MLKL Regulates Rapid Cell Death-independent HMGB1 Release in RSV Infected Airway Epithelial CellsFrontiers in Cell and Developmental BiologyMay 31, 2022Jennifer Simpson, Kirsten M. Spann, Simon PhippsGSK, 1:450), anti-human pRIPK3 (Ser227; CST, CST93654S; 1:800) anti-human RSV fusion protein (Abcam, ab20745; 1:500), anti-human cleaved caspase-3 (Asp 175) conjugated-488 (CST, CST9669; 1:200) and anti-human

(8) Multiple Respiratory Syncytial Virus (RSV) Strains Infecting HEp-2 and A549 Cells Reveal Cell Line-Dependent Differences in Resistance to RSV InfectionJournal of VirologyApril 13, 2022Anubama Rajan et al.diluted in PBS for 30 min at RT. RSV was detected using an anti-RSV antibody from Abcam (catalog no. ab20745) at 1:1000 dilution in 1% BSA overnight incubation at 4°C. Nuclei and actin were stained with 4′, 6′-diamidino-2-phenylindole

(9) Neutrophil Extracellular Traps Do Not Induce Injury and Inflammation in Well-Differentiated RSV-Infected Airway EpitheliumCellsFebruary 24, 2022Rosalie S. N. Linssen, Adithya Sridhar, Giulia Moreni, Nicole N. van der Wel, Job B. M. van Woensel, Katja C. Wolthers, Reinout A. BemFluor 555 Abcam Catalog ab218714) and for RSV (primary antibody goat anti- RSV 1:100, Abcam Catalog ab20745, with secondary donkey anti-goat Alexa Fluor 488, 1:1000, Abcam Catalog ab150129). Finally, inserts

(10) The Human Nose Organoid Respiratory Virus Model: an Ex Vivo Human Challenge Model To Study Respiratory Syncytial Virus (RSV) and Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Pathogenesis and Evaluate TherapeuticsmBioFebruary 22, 2022Anubama Rajan et al.0.2 μg/mL mucin 5AC (Invitrogen, catalog no. 45M1), goat anti-RSV IgG antibody (Abcam, catalog no. ab20745), and rabbit anti-SARS-CoV-2 S1 IgG antibody (Sino Biologicals, catalog no. 40150R007; BEI Resources,

(11) Paramyxovirus replication induces the hexosamine biosynthetic pathway and mesenchymal transition via the IRE1α-XBP1s arm of the unfolded protein responseAmerican Journal of Physiology-Lung Cellular and Molecular PhysiologySeptember 1, 2021Dianhua Qiao, Melissa Skibba, Xiaofang Xu, Roberto P. Garofalo, Yingxin Zhao, Allan R. Brasiermin, blocked in PBS + 5% FBS for 2 h, and incubated with goat polyclonal anti-RSV antibody (Cat. No. ab20745 at Abcam, 1:1,000 dilution) at 4°C overnight, followed by washing in PBS and then incubated at RT for

(12) Respiratory syncytial virus M2-1 protein associates non-specifically with viral messenger RNA and with specific cellular messenger RNA transcriptsPLOS PathogensMay 18, 2021Molly R. Braun, Sarah L. Noton, Emmeline L. Blanchard, Afzaal Shareef, Philip J. Santangelo, W. Evan Johnson, Rachel Fearnsprimary antibodies HA.11 (Biolegend, 901502), αM2-1 (Abcam, ab94805), 22k4, and 37M2, and αRSV (Abcam, ab20745) at a dilution of 1:500–1:1000 and LI-COR secondary antibodies (Li-Cor, 925–68072, 925–68073, and 925–32214)

(13) Human Respiratory Syncytial Virus Infection in a Human T Cell Line Is Hampered at Multiple StepsVirusesFebruary 2, 2021Ricardo de Souza Cardoso et al.(ab150115, Abcam) was diluted 1:2000. The antibodies for western blot experiments were goat anti-RSV (Abcam ab20745), diluted 1:1000; HRP-conjugated goat anti-rabbit (Invitrogen, Carlsbad, CA, USA), diluted 1:5000; and

(14) TMEM16A/ANO1 calcium-activated chloride channel as a novel target for the treatment of human respiratory syncytial virus infectionThoraxJanuary 1, 2021Pearson H, Todd EJAA, Ahrends M, Hover SE, Whitehouse A, Stacey M, Lippiat JD, Wilkens L, Fieguth HG, Danov O, et al.phosphate-buffered saline, 0.1% tween 20 detergent (PBS-T). Plaques were detected using goat α-HRSV (Abcam ab20745, 1:200) and HRP-conjugated rabbit α-goat (Sigma A8919, 1:5000) and imaged following incubation with

(15) Host Retromer Protein Sorting Nexin 2 Interacts with Human Respiratory Syncytial Virus Structural Proteins and is Required for Efficient Viral ProductionmBioOctober 27, 2020Ricardo S. Cardoso et al.Subsequently, the nitrocellulose membrane was incubated with the appropriate antibody, namely, goat anti-RSV (ab20745; Abcam), rabbit polyclonal anti-TGN46 (ABT95; Millipore), sheep polyclonal anti-TGN46 (A21448; Thermofisher),

(16) Polymerase-tagged respiratory syncytial virus reveals a dynamic rearrangement of the ribonucleocapsid complex during infectionPLOS PathogensOctober 8, 2020Emmeline L. Blanchard, Molly R. Braun, Aaron W. Lifland, Barbara Ludeke, Sarah L. Noton, Daryll Vanover, Chiara Zurla, Rachel Fearns, Philip J. Santangelodilution), mouse anti-RSV M2-1 (Abcam, Cat, No. ab94805, 1:1000 dilution), goat anti-panRSV (Abcam, Cat. No. ab20745, 1:1000 dilution), rabbit anti-FLAG (Cell Signaling Technology, Cat. No. 14793S, 1:1000 dilution), mouse

(17) The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins*Molecular & Cellular ProteomicsMay 1, 2020Olivier Touzelet, Lindsay Broadbent, Stuart D. Armstrong, Waleed Aljabr, Elaine Cloutman-Green, Ultan F. Power, Julian A. Hiscoxwith the following primary antibodies: rabbit anti-CSF3 (ab181053, Abcam), goat anti-hRSV proteins (ab20745, Abcam), mouse anti-hRSV matrix protein (M024, 1:500) and nucleoprotein (mab0015, 1:500), both kindly

(18) Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus productioneLifeJune 27, 2019Hu M, Schulze KE, Ghildyal R, Henstridge DC, Kolanowski JL, New EJ, Hong Y, Hsu AC, Hansbro PM, Wark PA, et al.Hansbro, and Peter AB Wark, University of Newcastle", Antibody,Goat polyclonal anti-RSV,Abcam,"Cat. #: ab20745, RRID:AB_777677",IF (1:400) Antibody,Mouse monoclonal anti-γ-tubulin,Proteintech,Cat. #: 66320–1-Ig,IF

(19) The viral protein corona directs viral pathogenesis and amyloid aggregationNature communicationsMay 27, 2019Ezzat K, Pernemalm M, Pålsson S, Roberts TC, Järver P, Dondalska A, Bestas B, Sobkowiak MJ, Levänen B, Sköld M, et al.Stripping buffer (LI-COR Biosciences), blocked for 1.5 h, and then incubated with anti-RSV antibody (ab20745, Abcam)65 at 1:500 dilution for 2 h at RT before probing with secondary antibody (donkey anti-goat,

(20) Effects of Alterations to the CX3C Motif and Secreted Form of Human Respiratory Syncytial Virus (RSV) G Protein on Immune Responses to a Parainfluenza Virus Vector Expressing the RSV G ProteinJournal of VirologyApril 1, 2019Bo Liang et al.the Odyssey imaging system (LI-COR). Primary antibodies included goat anti-RSV polyclonal antibodies (ab20745; Abcam), rabbit anti-HPIV3 antiserum (MS456) April 2019 Volume 93 Issue 7 e02043-18 jvi.asm.org 19 Downloaded

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Anti-Respiratory Syncytial Virus antibody from Abcam

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Anti-Respiratory Syncytial Virus antibody from Abcam

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